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Western blots with antibodies against Sst2p (gift from Dr. Orna Resnekov, Molecular Sciences Institute) and glucose-6-phosphate dehydrogenase. A, strains overexpressing Sst2p were immunoblotted with anti-Sst2p. An sst2-Δ strain and a strain expressing wild-type amounts of Sst2p (from the chromosome) are shown for control. Sst2p runs at ∼82 kDa. B, loading control using anti-glucose-6-phosphate dehydrogenase (G6PDH).
