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Signaling responses to α-factor in strains overexpressing Sst2p. FUS1-lacZ induction in response to α-factor binding in strains overexpressing Sst2p and expressing Ste2p from a repressed galactose-inducible plasmid (A4755) (A), the chromosome (A4757) (B), a CEN plasmid (A4744) (C), or a multicopy plasmid (A4746) (D) is shown. Closed circles represent strains expressing Sst2p from the chromosome, and open circles represent strains overexpressing Sst2p (under the ADH1 promoter).
