FIGURE 9.

Competition binding experiments between fluorescently labeled α-factor and unlabeled antagonist (see also Table 5). Competition binding is shown between 10 nm Lys⁷-NBD-α-factor and increasing concentrations of unlabeled d-Tyr³ in strains expressing the receptor Ste2p from a CEN plasmid (A4648, filled circles) or a multicopy plasmid (A4650, open circles). The ligands were mixed before adding to the sample. The symbols represent the mean for three independent experiments, and the error values are the standard error of the mean.