TABLE 1.
Binding and signaling parameters for α-factor at varying receptor expression levels
| STE2 allele | Yeast strain | Kd | Relative Bmaxa | EC50 | Relative maximal inductionb |
|---|---|---|---|---|---|
| nm | nm | ||||
| Chromosomal | A818 | 15 ± 11 | 1 | 23 ± 4 | 1 |
| Repressed GAL1-STE2 | A4754 | NDd | <0.2 × chromosomalc | 10 ± 2 | 0.6 ± 0.04 |
| CEN-STE2 | A4648 | 3 ± 0.3 | 2.5 ± 1 | 19 ± 8 | 1 ± 0.08 |
| Multicopy STE2 | A4650 | 7 ± 0.1 | 9 ± 4 | 38 ± 7 | 0.9 ± 0.07 |
| CEN-STE2-Δ305-431 | A4649 | 8 ± 0.3 | 17 ± 7 | 1.1 ± 0.3 | 1.4 ± 0.1 |
| Multicopy STE2-Δ305-431 | A4651 | 21 ± 1 | 55 ± 23 | 1.5 ± 0.1 | 1.2 ± 0.1 |
a The relative Bmax = Bmax of the respective strain/Bmax of the strain expressing full-length chromosomal Ste2p.
b The relative maximal induction = maximal induction of the respective strain/maximal induction of the strain expressing full-length chromosomal Ste2p.
c The data are from Gehret et al. (24).
d ND indicates not determined.