TABLE 4.
Ste2p agonist and antagonists
| Ligand type | Name | Sequence | Ki (CEN STE2)a | IC50 (CEN STE2)a | Ki (multicopy STE2)b | IC50 (multicopy STE2)b |
|---|---|---|---|---|---|---|
| nm | nm | nm | nm | |||
| Agonist | α-Factor | WHWLQLKPGQP(Nle)Yc | ||||
| Antagonist | dTA (des-Trp1,Ala3) | -HALQLKPGQP(Nle)Y | 12 ± 3 | 52 ± 11 | 14 ± 1 | 34 ± 3 |
| d-[r]Tyr3 | WH(dY)LQLKPGQP(Nle)Y | 5 ± 2 | 23 ± 6 | 10 ± 4 | 24 ± 9 | |
| dTH (des-Trp1,des-His2) | -WLQLKPGQP(Nle)Y | 6 ± 1 | 27 ± 4 | 25 ± 5 | 62 ± 13 |
a This was performed in yeast strain A4648.
b This was performed in yeast strain A4650.
c Nle refers to norleucine.