FIGURE 2.

PKH_020910 is produced during the asexual intraerythrocytic life cycle. A, immunoblot using anti-PKH_020910 antiserum probing extracts of either uninfected erythrocytes (Uninf.) or erythrocytes infected with P. knowlesi (Inf.). B, immunoblot using anti-spectrin antibodies of the same extracts as in A. The more intense band in the uninfected lane indicates that more cell equivalents were loaded in the lane containing the extract of uninfected erythrocytes. C, detection of PKH_020910 in infected erythrocytes by indirect immunofluorescence microscopy. Erythrocytes infected with late-stage P. knowlesi were stained with anti-PKH_020910 antiserum and DAPI to visualize the parasite nuclei. Staining is clearly visible within the parasites but is absent in uninfected erythrocytes. DIC, differential interference contrast.