FIGURE 5.

C-terminal extension of PFA0210c and its orthologues regulates phospholipid transfer. A, phospholipid transfer activity of full-length (FL) and truncated (Trunc.) PFA0210c, PKH_020910, and PCHAS_020730. Phospholipid transfer was measured using an activity assay that measures the transfer of radiolabeled phosphatidylcholine from a small population of donor vesicles to a larger population of acceptor vesicles. B, phospholipid transfer activity of full-length PFA0210c (FL), truncated PFA0210c (Trunc.), and PFA0210c where the last 48 amino acid residues have been replaced with the corresponding 48 residues from PCHAS_020730 (Pc C term.) or GFP (GFP-C term.). All assays were performed in triplicate. Error bars indicate standard deviation.