FIGURE 7.

Regulation of phospholipid transfer is required in vivo. Diagnostic PCR analysis of attempted integration of truncation plasmids designed to remove 8 codons (PKH_020910 468), 13 codons (PKH_020910 463), or 20 codons (PKH_020910 456) from the end of the gene encoding PKH_020910 or to remove internal codons 433–444 (PKH_020910 Δ12). Integration-specific PCR products were detected only in parasites that reconstituted the entire gene (PKH_020910 full length) and PKH_020910 Δ12. The PCR strategy and the primers used are described in Fig. 3A. All transfections were repeated a minimum of three times, each time yielding the same result.