FIGURE 1.

Primer extension reactions catalyzed by DNA polymerases on rG-, 8-oxo-rG, and 8-oxo-dG-modified DNA templates. Unmodified or modified 38-mer template DNA was annealed to an Alexa 546-labeled 10-mer primer. Reactions catalyzed by varying amounts of human pols α (6, 30, or 150 fmol) (A), κ (2, 10, or 50 fmol) (B), and η (2, 10, or 50 fmol) (C) were performed at 25 °C for 30 min in the presence of dNTPs. 13X indicates the position of the modification. % TLS indicates the percentage of the amount of primers beyond the lesion relative to the total amount of the primer.