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. 2016 Sep 22;291(46):24314–24323. doi: 10.1074/jbc.M116.738732

FIGURE 4.

FIGURE 4.

Effect of OGG1 on RNase H2-mediated ribonucleotide excision. A, the 5′-end 6-FAM-labeled DNA substrate containing rG or 8-oxo-rG (200 nm) was incubated with RNase H2 (0.5, 1.0, or 1.5 units) for 60 min at 37 °C in the absence (−) or presence (+) of OGG1 (750 nm). B, quantification of the product formed by RNase H2-mediated excision. ***, p < 0.001 when compared with “rG + RNase H2.” **, p < 0.01 between “8-oxo-rG + RNase H2” and “8-oxo-rG + RNase H2 and OGG1.” Values presented are the mean ± S.E. of four independent experiments. C, gel mobility shift assay to assess the substrate binding capacity of OGG1. The 5′-end 6-FAM-labeled DNA substrate containing 8-oxo-dG or 8-oxo-rG (50 nm) was incubated with varying amounts of OGG1 for 15 min on ice.