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Kinetic parameters for nucleotide insertion and chain extension reactions catalyzed by human DNA polymerase α
Frequencies of nucleotide insertion (Fins) and chain extension (Fext) were estimated by the following equation: F = (Vmax/Km)[wrong pair]/(Vmax/Km)[correct pair = dC:dG]. ND, reliable kinetic parameters could not be determined under steady-state conditions.
a Data were expressed as mean ± S.E. obtained from three independent experiments.
