Table 1.

Oligonucleotide primer sequences designed to validate the genomic sequence of Clostridium estertheticum DSM 8809.

Forward (F) and reverse (R) primer Sequence (5′→′)	Location/position (bp)	Location/sequenced region	Identity
F-ATG CAG GCA CAA CAA CCA TT	Chromosome	Chromosome	100%
R-AAT GCC TTT TCA AGC TCG CA	43,640–44,701	43,705–44,585
F-ATC CTC CGG TGG TTG TTG AT	Chromosome	Chromosome	100%
R-AAC GTT TGA CAC CAC TGC TC	1,220,689–1,221,824	1,220,800–1,221,760
F-CAG GTG GGA CGA ACT CTT CT	Chromosome	Chromosome	100%
R-CTC CTG TCC TGT TGC AAA CC	2,409,276–2,410,209	2,409,328–2,410,147
F-CTT CGA ATC TGT TGC AGC GA	Chromosome	Chromosome	100%
R-GCG ACA CTG ATG CCT ACA AG	2,794,994–2,795,848	2,795,033–2,795,834
F-TTT ACA ACC GCT GCT CCA AC	Chromosome	Chromosome	99%
R-GGA GGG GTA CGT GAT GGT AG	3,170,935–3,171,943	3,171,004–3,171,890	(WGS)
F-CGA GTG TCT GGT TTG CAA CA	Chromosome	Chromosome	100%
R-ATG GTA GGG GTT GTT GGA GG	3,671,665–3,672,574	3,671,778–3,672,460
F-CGC CTC ACT TAA AGA ACC GG	Chromosome	Chromosome	100%
R-TTC CGT CGG TTA GTG GTT CA	4,702,142–4,703,114	4,702,215–4,703,038
F-GAG TTA AGT GGA GGG GAG CA	Chromosome	NA
R-TGT GAG CAT CGT CCT GTG TA	683,264–686,661
F-TGG CAT CCA ACG ACA ATT GT	Chromosome	NA
R-AGA GGC ATT CGA AGT TGA AGT	1,722,582–1,725,596
F-ACC TGC GTG CTA AGT AAC CT	Chromosome	NA
R-AGT GGA TGC TAC AAG GCT CA	4,001,142–4,004,064
F-TAA GAT GAT GGG AGG AGC GG	Plasmid	Plasmid	100%
R-GAT TGC CAC TGT TTG CTT GC	675–1233	685–1,219
F-CTG AAA ATG CAG CCG TTG AT	Plasmid	Plasmid	100%
R-TGC AAA TAG TAC CGT TGG CA	8,101–8,757	8,113–8,743
F-GAC GAT GCC AGT GAA ACG AT	Plasmid	Plasmid	100%
R-ACG GTC ATT GCT CCT GAA GA	14,519–15,129	14,536–15,115