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. 2016 Nov 11;6:36832. doi: 10.1038/srep36832

Figure 5. Translational inhibition abolishes the OCTR-1-controlled innate immune responses.

Figure 5

(A) qRT-PCR of abu-1, abu-7, abu-8, abu-12, abu-13, abu-14, and abu-15 expression in wild-type animals with or without exposure to G418 and in octr-1(ok371) animals exposed to G418. n = 3; bar graphs correspond to mean ± SEM. t-tests were performed between N2 + G418 and octr-1 + G418. *indicates significant difference. (B) qRT-PCR of abu-1, abu-7, abu-8, abu-12, abu-13, abu-14, and abu-15 expression in wild-type animals with or without exposure to Hygrocymin B and in octr-1(ok371) animals exposed to Hygromycin B. n = 3; bar graphs correspond to mean ± SEM. t-tests were performed between N2 + G418 and octr-1 + G418. *indicates significant difference. (C) Wild-type and octr-1(ok371) animals were exposed to NGM/E. coli OP50 (control) or NGM/E. coli OP50 containing G418 or Hygromycin B, and scored for survival over time. WT + No inhibitor versus octr-1(ok371)+No inhibitor: p = 0.7521; WT + G418 versus octr-1(ok371)+G418: p < 0.0001; WT + Hygromycin B versus octr-1(ok371)+Hygromycin B: p < 0.0001. Shown is a representative assay of two independent experiments. n = 45 young adult animals per strain. p values < 0.05 are considered significant.