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. 2016 Nov 11;6:36835. doi: 10.1038/srep36835

Figure 4. Cooling of nsEP-exposed cells induces caspase 3/7 activation and PARP cleavage.

Figure 4

U-937 cells were exposed to 50, 300 ns pulses (100 Hz, 7 kV/cm) and immediately incubated at 37 °C, or placed on ice for 30 min and then in the incubator. Panels (A,B) show, respectively, the activity of caspase 3/7 and cell survival at 4.5 hr after nsEP with or without cooling. For a positive control, apoptosis was induced by incubation with 10 μm staurosporine for 4.5 hr. Panel C shows a representative Western blot for intact and cleaved PARP (116 and 89 kDa, respectively) and the quantification of the cleaved fraction at 4.5 hr after nsEP exposure.; For a positive control, apoptosis was induced with 10 μm staurosporine. Mean +/− s.e. n = 6–9 (A,B) or n = 3 (C). *p < 0.001 for the difference of nsEP + cooling from nsEP + 37 °C.