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. 2016 Nov 11;6:36829. doi: 10.1038/srep36829

Figure 2. Transduction of EPCR expression in MPM cells promotes apoptosis.

Figure 2

Monolayers of REN MPM cells that lack EPCR expression were stably transfected with empty vector or EPCR expression vector. REN and REN(+EPCR) cells were treated with TNFα (10 ng/ml), IFNγ (100 U/ml) or TNFα + IFNγ (10 ng and 100 U/ml) or none (untreated/control) in 10% v/v serum-containing medium. After 72 h, cells were harvested and processed for TUNEL assay. The samples were analyzed using BD FACS Calibur. (a) Flow cytometry data from a typical experiment. (b) Mean data from three independent experiments. ***p < 0.001. (c) REN and REN(+EPCR) cells were treated with TNFα (10 ng/ml), IFNγ (100 U/ml) or TNFα + IFNγ (10 ng and 100 U/ml) for 24 h and cell extracts were subjected to western blot analysis and probed for apoptotic markers, cleaved caspase 3 (cl. Casp 3), cleaved poly (ADP-ribose) polymerase (cl. PARP), and p-BAD.