Figure 4. Ad.EPCR treatment curtails the progression of MPM in the mouse model.
Nude mice (BALB/c, NU/J) were implanted with 1 × 106 REN MPM cells in the thoracic cavity. On the 11th day (d 11) following the tumor cell implantation, a group of mice was killed to monitor the establishment of MPM tumors in the thoracic cavity. Then the remaining mice were divided randomly into three groups, and each group of mice was injected intrapleurally with either sterile PBS (100 μl), the control adenovirus (Ad.Con), or EPCR adenovirus (Ad.EPCR) (2 × 109 pfu in 100 μl PBS, once every three days). At the end of 30 days (d 30), mice were euthanized, and tumor statistics were recorded. (a) tumor count; (b) tumor volume; (c) tumor burden; (d) a representative photograph showing differences in tumor growth in mice treated with PBS, control adenovirus or EPCR adenovirus following REN MPM cell implantation for 10 days. Arrows indicate tumors. A total of three independent experiments were conducted with a total of 16–18 mice/group. *p < 0.05; **p < 0.01; ***p < 0.001.