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. 2016 Nov 11;6:36843. doi: 10.1038/srep36843

Figure 5. CUDR plus IL6 enhances Stat3 expression and phorsphorylation through pNF-κB.

Figure 5

(a) Chromatin Immunoprecipitation (CHIP) with anti- pNF-κB followed by PCR with Stat3 promoter primers in hepatocyte-like stem cells treated with IL6 and transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pGFP-V-RS, pGFP-V-RS-CUDR. IgG CHIP as negative control. Stat3 promoter DNA as INPUT. (b) Stat3 promoter luciferase activity assay. Each value was presented as mean ± standard error of the mean (SEM). **P < 0.01. (c) The analysis of Stat3 expression by RT-PCR with Stat3 primers. β-actin as internal control. (d) Western blotting with anti-Stat3 and pStat3 in hepatocyte-like stem cells treated with IL6 and transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pGFP-V-RS, pGFP-V-RS-CUDR. β-actin as internal control.