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. 2016 Nov 11;6:36803. doi: 10.1038/srep36803

Figure 4. In vivo titration of miR-208a increases cardiac phospho-substrates by suppressing PDE4D.

Figure 4

Experimental timeline for transgenic mice (A) and summary of titration of miR-208a levels in vivo (B). Two sites showing alignment highlighting the seed match sequences of mouse PDE4D 3′UTR (red) that is complementary for the seed sequences of miR-208a (C). The PDE4D content (D,E), the phosphoprotein content for cTnI (F,G) and PLN (H,I) are shown with representative Westerns and summary plots. Data are shown as the means +/− SEM. N = 3 *P < 0.05. Abbreviations, (+) Dox = dox treated, (−) Dox = no dox, (+/−) Dox = dox treated for 3 weeks and dox removed for 2 days.