Figure 3. CDK inhibition induces neutrophil apoptosis at the site of tailfin injury in vivo.

(a) TSA/TUNEL staining was carried out on Tg(mpx:EGFP)ⁱ¹¹⁴ zebrafish following tailfin transection and either DMSO [i] or AT7519 [ii] treatment, labelling neutrophils in green, and apoptotic cells in red, with representative images shown. (b) Apoptotic neutrophils (double positive red and green) are shown by the arrows. The absolute number of double positive cells were quantified at 12 hpi. ≥16 embryos per group in 3 independent experiments. *p < 0.05 12 h DMSO vs 12 h AT7519, unpaired t test. n/s: not significant. Data expressed as ±S.E.M.