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. 2016 Nov 11;6:36890. doi: 10.1038/srep36890

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Copyright © 2016, The Author(s)

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(A) The amount of each HN protein was determined by western blot analysis at 24 h post-transfection of Vero cells by using anti-SG10 serum. HAd (B) and NA activities (C) were examined in Vero cells transfected with the 0.5 μg each of pCI-HN mutant plasmids. HN protein expression level, HAd and NA activities of these HN mutants are expressed as percentages of the values for expressed HN571 protein (pCI-HN571), which are deemed to be 100%. Each bar represents the mean and standard deviation of three independent experiments. Asterisks indicate the significance of the differences between the biological activity of an HN protein mutant and that of the HN571 protein. P values were calculated with Tukey’s test (95% confidence levels). *p < 0.05, significant; **p < 0.01, very significant.