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. 2016 Nov 11;6:36305. doi: 10.1038/srep36305

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Proteomic identification of the C-myc-mdig-centered protein interaction network following c-myc and mdig pull-downs. All determined proteins, excluding mdig and c-myc themselves, are categorized as Myc-only (blue), Mdig-only (green) and Shared (cyan) groups while total numbers of each group are listed in the Venn diagram; (B) A chart summarizes all the unique peptide sequences of mdig detected by mass spectrometry in c-myc pull-downs; (C) Co-immunoprecipitation (co-IP) assay shows direct physical binding of c-myc and mdig in NCI-H929 and MM1S cells; (D) Summaries of top biological processes represented by the interaction partners of c-myc and mdig. All determined subjects are interrogated by Gene Ontology database and are sorted based on biological processes they participate in.