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. 2016 Nov 8;7:13234. doi: 10.1038/ncomms13234

Figure 3. H2O2-induced potentiation of sensory behavior and ASH neuron function requires the p38/MAPK PMK-1.

Figure 3

(a) H2O2-induced behavioral potentiation requires PMK-1. In pmk-1(km25) but not pmk-3(ok169) mutant worms, H2O2 treatment failed to promote osmotic avoidance behavior. This defect was rescued by a transgene expressing pmk-1 cDNA in ASH neurons driven by the sra-6 promoter. n≥20; *P<0.05, **P<0.005, ***P<0.0005 (ANOVA); Error bars: s.e.m. (b) H2O2-induced behavioral potentiation does not require ERK (MPK-1 and MPK-2) or JNK (JNK-1). mpk-1(tm3476), mpk-2(tm3859) and jnk-1(gk7) mutant worms were tested. n≥10; ***P<0.0005 (ANOVA); Error bars: s.e.m. (ce) H2O2-induced potentiation of ASH sensory response requires PMK-1. In pmk-1 mutant worms, H2O2 treatment can no longer promote ASH calcium response to glycerol stimulus (c). This defect was rescued by a transgene expressing pmk-1 cDNA in ASH neurons driven by the sra-6 promoter (d). Shades along the traces in (c) and (d) represent error bars (s.e.m.). (e) summarizes the data in (c) and (d). n≥9; **P<0.005 (ANOVA); Error bars: s.e.m.