Figure 2. TSC22D4 regulates systemic glucose homeostasis.

(a) Serum glucose levels of 16 h fasted control or TSC22D4 miRNA AAV–injected C57Bl/6 mice 6 weeks after virus injection (means±s.e.m., n=5). (b) Serum insulin levels in same mice as in a. (c) HOMA-IR index of same mice as in a. (d) Glucose tolerance test in same mice as in a, 2 weeks after miRNA injection. Glucose was injected i.p. at a concentration of 1 g glucose kg⁻¹ body weight (means±s.e.m., n≥6). (e) Serum glucose levels of 16 h fasted control or TSC22D4shRNA adenovirus–injected C57Bl/6 mice 7 days after virus injection (means±s.e.m., n≥6). (f) Serum insulin levels in same mice as in e. (g) HOMA-IR index in same mice as in e. (h) Serum insulin levels in empty control (control cDNA) or Flag- TSC22D4 (TSC22D4 cDNA) cDNA adenovirus-injected random fed C57Bl/6 mice that were fed either a LFD or HFD for 11 weeks (means±s.e.m., n=5). (i) HOMA-IR index in same mice as in h. (j) Serum glucose levels in same mice as in h. Statistical analysis: Student's t-test, *P≤0.05; **P≤0.01; ***P≤0.001.