Table 1. Levels of oxidized haemoglobins in uninfected erythrocytes.
| Erythrocyte variant | Pretreatment reagent | Oxy-Hb (%) | Met-Hb (%) | Irreversibly oxidized Hb (%) |
|---|---|---|---|---|
| HbAA | — | 94.0 | 1.0 | 5.0 |
| HbAS | — | 85.6 | 2.3 | 12.2 |
| HbAC | — | 83.5 | 3.9 | 12.6 |
| HbF | — | 84.7 | 3.1 | 12.2 |
| HbAA | H2O2 | 78.1 | 1.3 | 20.6 |
| HbAA | tBOOH | 77.7 | 5.4 | 16.9 |
| HbAA | Menadione | 70.1 | 7.3 | 22.0 |
Met-Hb, methaemoglobin; Oxy-Hb, oxy-haemoglobin.
Where indicated HbAA erythrocytes were pretreated with H2O2, tert-butyl hydroperoxide (tBOOH) or menadione. The experimental uncertainty of the determinations is <1.5% and the mean of three independent biological replicates, using blood from three different donors, is shown.