Fig. 3.

Effect of miR-375 on docetaxel resistance in xenograft mice. Nude mice were inoculated with PC-3 cells transfected miR-375 or control vector to allow tumor development. Mice were intraperitoneally injected with 10 mg/kg docetaxel once a week. a Tumor volumes in the docetaxel-treated mice are increased with time, in particular the xenograft tumors overexpressing miR-375. b Xenografts with empty control vector were significantly smaller than the miR-375 overexpression group. Representative tumors from each group are shown. c Tumor weights at the end of the treatment period (28 days after first docetaxel injection) were significantly higher in miR-375 group. Data are represented as the mean ± SD. (n = 6, P <0.05). d Caspase-3 immunohistochemistry analyses of xenograft tumors with empty control vector or miR-375 lentivirus vector after docetaxel treatment. Under docetaxel (DTX) treatment, less apoptotic cells were observed in miR-375 overexpressed xenograft tumors than miR-375 vector controls