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. 2016 Nov 10;15:70. doi: 10.1186/s12943-016-0556-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Expression of miR-375 and its target genes (YAP1 and SEC23A) in PC cells or xenograft tumors. a qRT-PCR analysis showed that YAP1 and SEC23A expressions were downregulated after miR-375 transfection in PC-3 cells. * indicates statistically significant reduction. b Western blots showed suppression of YAP1 and SEC23A proteins in the miR-375-transfected PC-3 cells. c qRT-PCR analysis showed that YAP1 and SEC23A expression levels were lower in xenografts overexpressing miR-375. d Western blots showed suppression of YAP1 and SEC23A proteins in xenograft tumors overexpressing miR-375. β-actin served as a loading control to normalize protein signal intensity