Fig. 2.

IBs from wt and ΔcobB cells contain acetylated VP1GFP. IBs were isolated after 24 h of IPTG induction from the bacteria cultivated, as described in the legend to Fig. 1. The whole-cell extracts and purified IBs were submitted to SDS-PAGE and immunodetection using anti-GFP (Sigma) and anti-acetyl lysine antibodies (Abcam). IBs isolated from equal amounts of cells were loaded onto the gel