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. 2016 Nov 10;8:56. doi: 10.1186/s13099-016-0133-1

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Flow cytometric analysis of T lymphocytes. Flow cytometric analysis of the percentage of CD4+ intraepithelial lymphocytes (IEL) in caecum of broiler-type (BT) (a, c) and layer-type (LT) birds (b, d) fed with either broiler feed (bf) or layer feed (lf). Birds had been C. jejuni-inoculated at 1 (Exp. 1) (a, b) and 22 (Exp. 2) (c, d) days post hatch. CD4+ T cells were gated within the CD3+ IEL population. Asterisk letters indicate significant differences between C. jejuni-inoculated (C. jejuni) and C. jejuni-free control (con) groups at the indicated days post C. jejuni inoculation (n = 6/group, p < 0.05)