Table 2.
In vivo validation of endothelium-related genes highly expressed in BMECs compared to SCMECs
| Gene | Probe | MA (in vitro) | RT-qPCR (in vivo) |
|---|---|---|---|
| Bgn | A_43_P11812 | 22.9 | 1.5 |
| Col3a1 | A_44_P146518 | 20.9 | 2.3 |
| Col1a1 | A_44_P238421 | 17.2 | ND |
| Col1a2 | A_43_P12783 | 13.6 | 1.9 |
| Slit3 | A_44_P1024315 | 12.8 | ND |
| Mgp | A_42_P588944 | 12.3 | ND |
| Cldn9 | A_44_P419898 | 10.0 | 46.2 |
| Spp1 | A_44_491796 | 4.9 | 0.5 |
| Ctgf | A_42_P484738 | 4.8 | 2.1 |
| Tgfb2 | A_44_P246538 | 2.5 | 1.9 |
Ratio of the values in BMEC versus SCMEC monolayers (MA microarray) and freshly extracted BMVs versus SCMVs (RT-qPCR)