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. 2016 Nov 1;36(11):652–665. doi: 10.1089/jir.2016.0051

FIG. 3.

FIG. 3.

Type I IFNs induced by SeV do not protect against SeV-induced CPE. (A) Supernatants from primary monocytes that were either UI or infected with SeV (150 HA U/mL) for 24 h were treated with β-propiolactone to inactivate the virus and then used to pretreat fresh U937 cells for 24 h. The U937 cells were then infected with SeV (150 HA U/mL) for 72 h and cell viability was measured via MTT assay. Results are shown as the % of cell control. Supernatants from (B) U937, (C) Namalwa, or (D) A549 cells that were UI or infected with SeV for 16 h were treated with β-propiolactone to inactive the virus and then used to pretreat fresh U937 cells for 24 h. The cells were then infected with SeV (150 HA U/mL) for 72 h and cell viability was measured via MTT assay. Results are shown as the % of cell control and are representative of 3 independent biological replicates. CPE, cytopathic effects.