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. 2016 Nov 15;25(22):1709–1720. doi: 10.1089/scd.2016.0216

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Copyright 2016, Mary Ann Liebert, Inc.

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FIG. 3. — Validation of top ranked protocols in alginate beads. Expansion of CD133⁺ cells in alginate beads using the top ranking protocols was evaluated using large particle flow cytometric analysis (COPAS). (A) Percentage of beads containing cells positive for Ki67 and CD133 with total fluorescence values above the threshold is shown. Values are mean ± SEM for two to four technical replicates with a pool of three independent CB donors. Statistical analysis was performed in GraphPad Prism using one-way ANOVA and Tukey's ad hoc test, and P < 0.05 was considered as statistically significant (*P < 0.05). (B) Flow cytometry plots from large particle sorter (COPAS) showing the gating strategy and the distribution of beads in two top ranking protocols (379 and 747 as a serial or mix protocol). ANOVA, analysis of variance.