FIG. 5.

Expansion of human CB CD133+ cell subsets on 3D nanofiber scaffolds. Histograms show the fold change in phenotypically defined HSC (A), MPP (B), CLP (C), CMP (D), MEP (E), and GMP (F) after 8 days of culture commencing from 2,500 human CB CD133⁺ cells plated onto 3D nanofiber scaffolds at day 0. Cytokines were added by medium change for the protocols shown (379, 747, 245, 241, and basal) at specific days of culture as described in Table 2. Individual biological replicates and average values are shown. Values are mean ± SEM for three to five donors (biological replicates). Statistical analysis was performed in GraphPad Prism using one-way ANOVA and Tukey's ad hoc test and P < 0.05 was considered as statistically significant (**P < 0.01, *P < 0.05).