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Sizing and partial characterization of randomly selected recombinants from the P. hawaiensis BAC library. Eighteen clones were selected at random from the library before arraying. DNA was isolated using standard miniprep procedure, subjected to NotI digestion and analyzed by PFGE as described in Methods. Low-range PFGE molecular weight markers (New England Biolabs) are denoted by M and their sizes are given in kb. Miniprep for the BAC clone analyzed in lane 16 did not yield any DNA.
