Table 4.
Comparison of UL10:UN10 ratio in healthy human subjects between current study and published literature reports using various methods for quantification of UL and UN in plasma or serum.
| Reference | UL10:UN10 ratio | Blood anticoagulant | Extraction solvent | Plasma volume, μL | Internal standard | Detection technique |
|---|---|---|---|---|---|---|
| Takada et al.19 | 1.1 | Heparin | EtOH/hexane | 1000 | CoQ9 | Amperometric |
| Okamoto et al.20 | 6.7 | Heparin | Hexane | 200 | CoQ9 | Amperometric |
| Wakabayashi et al.12 | 1.9 | Serum | Hexane | 100 | CoQ9 | Amperometric |
| Finckh et al.11 | 19.0 | Heparin | Hexane | 10 | CoQ9 | Coulometric |
| Lagendijk et al.17 | 16.5 | EDTA | 1-propanol | 300 | N/A | Coulometric |
| Yamashita et al.21 | 21.7 | Heparin | MeOH/hexane | 50 | CoQ9 | Amperometric |
| Kaikkonen et al.22 | 7.8 | Heparin/EDTA | MeOH/hexane | 50 | CoQ7/CoQ9 | Coulometric |
| Wang et al.23 | 13.3 | EDTA | EtOH | 100 | N/A | Amperometric |
| Menke et al.24 | 7.3 | EDTA | Hexane | 100 | UL9/UN9 | Coulometric |
| Tang et al.15 | 26.0 | Heparin | 1-propanol | 100 | UL9/UN9 | Coulometric |
| Ruiz-Jiménez et al.8 | 13.8 | Heparin | hexane | 100 | UL9/UN9 | LC-MS/MS |
| Miles et al.18 | 41.0 | Heparin | 1-propanol | 100 | UL9/UN9 | Coulometric |
| Franke et al.25 | 19.0 | Heparin | Hexane | 200 | UL9/UN9 | Coulometric |
| Matsuzaki et al.26 | 24.8 | Serum | MeOH/hexane | 50 | CoQ9 | Amperometric |
| Current study | 41.7 | Heparin | 1-propanol | 100 | UL9/UN9 | LC-MS/MS |
CoQ9: coenzyme Q9; LC-MS/MS: liquid chromatography-tandem mass spectrometry.