FIG 3.

Mutation of the GARP complex impairs production of IEVs. (A) WT and MU MEFs were infected with VACV-A5-EGFP (green) at 5 PFU/cell for 8 h and then fixed and labeled with anti-B5 antibody (red), phalloidin (orange), and DAPI (blue). Images are representative confocal sections acquired with a Zeiss LSM 710 confocal microscope. (B) 3D reconstruction of z-stack confocal series of WT and MU MEFs infected with VACV-EGFP. (Top) DAPI (blue), VACV cores (green), B5 (red), and actin (phalloidin, gray); (bottom) the same images from the top panels with the red (B5) channel removed.