FIG 4.

Mutation of the GARP complex disrupts the wrapping of IMVs, resulting in aberrant B5 accumulations. Imaris image analysis software (Bitplane) was used to quantitate the number of B5-labeled puncta in each cell (A), the percentage of B5-labeled puncta in a cell which colocalized with an EGFP-labeled core (B), the percentage of B5 puncta which colocalized with F13 (C), and the diameter of B5-labeled puncta in WT and MU MEFs (D). Data represent the averages and SEMs from an analysis of 49 cells (24 WT and 25 MU cells) from three independent experiments. ***, P < 0.001 by two-tailed Student's t test. (E) The diameter of B5-labeled puncta in MU MEFs was compared. The diameter of normal B5-labeled puncta which were associated with an EGFP-labeled core and the diameter of abnormal B5-labeled puncta with no associated core were compared. Data represents the averages and SEMs from an analysis of 25 MU cells from three independent experiments. ***, P < 0.001 by two-tailed Student's t test.