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. 2016 Oct 28;90(22):10170–10181. doi: 10.1128/JVI.01396-16

FIG 10.

FIG 10

Effect of the gD-null mutation on projection formation at the plasma membrane in HSV-1-infected cells. (A) Vero cells were mock infected or infected with wild-type HSV-1(F), F-BAC gD−, or F-BAC gD−-repair at an MOI of 5, harvested at 18 h postinfection, lysed, and analyzed by immunoblotting with antibodies against the indicated proteins. (B) Vero cells were mock infected or infected with wild-type HSV-1(F), F-BAC gD−, or F-BAC gD−-repair at an MOI of 5, fixed at 18 h postinfection, and examined by transmission electron microscopy. Bars, 2 μm. (C) Infected cells were examined by transmission electron microscopy as described in the legend to panel B, and the number of projections at the plasma membranes of 30 cells was quantitated. Statistical analysis was performed by one-way analysis of variance with the Tukey test. The asterisks indicate statistically significant differences, as follows: *, P < 0.05.