TABLE 2.
Effect of gD-null mutation on distribution of virus particles in infected Vero cells
| Virus | Mean ± SE % virus particles in each morphogenetic stagea |
Total no. of particles counted/total no. of cells | ||||
|---|---|---|---|---|---|---|
| Nucleocapsids in nucleus | Enveloped virions in perinuclear space | Nucleocapsids in cytoplasm | Enveloped virions in cytoplasm | Extracellular enveloped virions | ||
| HSV-1(F) | 51.7 ± 11.8 (800) | 8.0 ± 3.6 (124) | 7.8 ± 1.5b (121) | 3.8 ± 0.5 (59) | 30.3 ± 3.2b (876) | 1,546/12 |
| F-BAC gD− | 58.5 ± 10.2 (683) | 6.4 ± 1.3 (75) | 19.2 ± 4.1 (224) | 4.6 ± 0.9 (52) | 18.9 ± 3.0 (508) | 1,167/12 |
| F-BAC gD−-repair | 51.7 ± 9.7 (652) | 7.0 ± 2.9 (88) | 6.8 ± 2.1b (86) | 5.0 ± 1.9 (63) | 25.6 ± 3.4b (783) | 1,261/12 |
a
Numbers in parentheses are the numbers of virus particles.
b
The difference between this value and that for the F-BAC gD− mutant was statistically significant according to one-way analysis of variance with the Tukey test (P < 0.05).