FIG 2.

SIVmac Vif, but not HIV-1 Vif, counteracts primate A3Cs. (A and C) 293T cells were transfected with expression plasmids for SIVmacΔVif-Luc or SIVmac-Luc (A) or SIVmacΔVif-Luc or SIVmacΔVif-Luc plus HIV-1 Vif (C), together with expression plasmids for hA3G and primate A3Cs. pcDNA3.1(+) was used as a control (vector). After normalizing for reverse transcriptase activity, viral infectivity was determined by quantification of luciferase activity in 293T cells. (B and D) Lysates of SIVmac producer cells were used to detect the expression of A3s, SIVmac capsid (p27), or HIV-1 Vif by anti-HA, anti-p27, or anti-V5 antibody, respectively. Tubulin served as a loading control. Encapsidation of A3s into SIVmac was detected by anti-HA antibody. (E) Primate A3Cs and HIV-1 Vif (without tag) expression plasmids were cotransfected into 293T cells. The expression of A3C and HIV-1 Vif was detected by anti-HA and anti-Vif antibodies. (F) Expression plasmids for hA3C and cpzA3C were cotransfected with HIV-1 Vif or SIVcpz Vif into 293T cells. The expression of A3C and Vif was detected by anti-HA and anti-V5 antibodies. Pts, Pan troglodytes schweinfurthii. cps, counts per second. VLP, virus-like particle. Asterisks represent statistically significant differences: ***, P < 0.001; ns, no significance (Dunnett's t test).