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. 2016 Oct 28;90(22):10193–10208. doi: 10.1128/JVI.01497-16

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FIG 3 — Sensitivity of rh/hA3C and h/smmA3C chimeras to HIV-1 Vif-mediated depletion. (A and C) Schematic structure of rh/hA3C and h/smmA3C chimeras. Amino acid positions 120, 150, and 190 in hA3C are indicated. (B and D) Immunoblots of protein lysates of cotransfected cells displaying the A3's sensitivity to HIV-1 Vif-triggered degradation. The expression of A3s and HIV-1 Vif were analyzed by using anti-HA and anti-V5 antibodies. Tubulin served as a loading control. +, with HIV-1 Vif; −, without HIV-1 Vif.