FIG 4.

Identification of determinants in rhA3C and smmA3C that confer resistance to HIV-1 Vif. (A) The schematic structure of rhA3C and smmA3C mutants. The numbers represent amino acid positions in A3C. +, sensitive to HIV-1 Vif-induced degradation; −, resistant to HIV-1 Vif-induced degradation. (B and D) hA3C, rhA3C, smmA3C, or A3C mutants were cotransfected with HIV-1 Vif into 293T cells. A3, HIV-1 Vif, and tubulin were detected by using anti-HA, anti-V5, and anti-tubulin antibodies, respectively. (C) Schematic structure of hA3C mutants.