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HIV-1 Vif binding to A3C and A3F mutants. (A) Coimmunoprecipitation of HIV-1 Vif SLQ/AAA with A3C and A3F mutants. Protein cell lysates (Input) and immunoprecipitated complexes (IP) were analyzed by immunoblotting with anti-Vif for HIV-1 Vif or anti-HA for A3. (B) ImageJ was used to evaluate the band density of A3C and Vif from immunoprecipitation. The ratio of Vif to A3 was calculated as A3-Vif binding activity. WT A3C-Vif and WT A3F-Vif binding activity was set as 100%.
