FIG 8.

Identification of important residues in subtype F-1 Vif that determine its counteraction of hA3C/F. (A) Schematic structure of F-1 Vif mutants. The N-terminal hA3H hapII box and C-terminal hA3C/F box are shown. (B, C, and D) hA3C and hA3F were transfected into 293T cells together with B-NL4-3 Vif or with F-1 Vif or F-1 Vif mutant. A3 and Vif were detected by using anti-HA and anti-Vif antibodies. Tubulin served as a loading control. (E) The structures of wild-type F-1 Vif and F-1 Vif mutants were modeled by SWISS modeling. The internal interaction between residues 171 and 167 was analyzed by PyMOL and is shown as a red dashed line.