FIG 9.

Structural differences between B-NL4-3 Vif and F-1 Vif. (A and C) hA3C and hA3F or hA3G expression plasmids were cotransfected together with B-NL4-3 Vif and its mutants. A3 and Vif were detected by using anti-HA and anti-Vif antibodies. Tubulin served as a loading control. (B) The structures of B-NL4-3, F-1 Vif, and their variants were modeled by SWISS modeling. The internal interaction between residues 171, 167, and 101 were analyzed by PyMOL, and the distances between interacting side chains are between 3.4 and 3.8 Å. The internal interaction is shown as a red dashed line. (D) Structure analysis of HIV-1 Vif (PDB entry 4N9F) for hA3C/hA3F interaction sites. Three discontinuous HIV-1 Vif motifs (F1 box, F2 box, and F3 box) that interact with hA3C/F are shown in red. 167R that contacted the F3 box is shown in magenta.