TABLE 1.
Primers used in this study
| Primer name | Product | Sequence (5′–3′) |
|---|---|---|
| PrmrRhAPO3C | TATAAGCTTTGAAGAGGAATGAATCCACAGATCAGAAACC | |
| PrmrRhAPOend | AGCTCGAGTCAAGCGTAATCTGGAACATCGTATGGATACTGAAGAATCTCCCGTAGGCG | |
| CM28 | AGCTCGAGTCAAGCGTAATCTGGAACATCGTATGGATACTGGAGACTCTCCCGTAGCCTT | |
| PrmrRhehu 9.1 | rh/hA3C9 | AAGGGCTCCAAGATGTGTAC |
| PrmrRhehu 9.2 | GTACACATCTTGGAGCCCTT | |
| PrmrRhehu 11.1 | rh/hA3C11 | CCACCTCCCCTGCACA |
| PrmrRhehu 11.2 | TGTGCAGGGGAGGTGG | |
| PrmrRhehu 1.1 | rh/hA3C13 | TCCACGGTGAAGCACAGC |
| PrmrRhehu 1.2 | GCTGTGCTTCACCGTGGA | |
| PrmrRhehu 3.1 | GTGAGATTCACGTTGCTGTGCCTGGCCAGGAACTTGG | |
| PrmrRhehu 3.2 | CCAAGTTCCTGGCCAGGCACAGCAACGTGAATCTCAC | |
| PrmrRhehu 1.1 | rh/hA3C15 | TCCACGGTGAAGCACAGC |
| PrmrRhehu 1.2 | GCTGTGCTTCACCGTGGA | |
| PrmrRhehu 11.1 | CCACCTCCCCTGCACA | |
| PrmrRhehu 11.2 | TGTGCAGGGGAGGTGG | |
| PrmrRhehu 1.1 | rh/hA3C17 | TCCACGGTGAAGCACAGC |
| PrmrRhehu 1.2 | GCTGTGCTTCACCGTGGA | |
| PrmrRhehu 9.1 | AAGGGCTCCAAGATGTGTAC | |
| PrmrRhehu 9.2 | GTACACATCTTGGAGCCCTT | |
| PrmrRhehu 7.1 | rh/hA3C31 | AGGAAGCACCTTTCTGCATG |
| PrmrRhehu 7.2 | CATGCAGAAAGGTGCTTCCT | |
| PrmrRhehu 3.1 | GTGAGATTCACGTTGCTGTGCCTGGCCAGGAACTTGG | |
| PrmrRhehu 3.2 | CCAAGTTCCTGGCCAGGCACAGCAACGTGAATCTCAC | |
| HuA3C-EcoRI-F | ATGAATTCGCCACCATGAATCCACAGATCAGAAAC | |
| HA-NotI-R | ATGCGGCCGCTCAAGCGTAATCTGGAACATC | |
| HuSmmC1 + 2-F | smm/hA3C1 + 2 | TCGGAACGAAACTTGGCTGTGCTTC |
| HuSmmC1 + 2-R | GAAGCACAGCCAAGTTTCGTTCCGA | |
| HuSmmC3 + 4-F | smm/hA3C3 + 4 | CCATTGTCATGCAGAAAGGTGCTTCCTCTC |
| HuSmmC3 + 4-R | GAGAGGAAGCACCTTTCTGCATGACAATGG | |
| HuSmmC5 + 6-F | smm/hA3C5 + 6 | TGCAGGGGAGGTGGCCGAGTTCCTGGCCAGG |
| HuSmmC5 + 6-R | CCTGGCCAGGAACTCGGCCACCTCCCCTGCA | |
| HuSmmC7 + 8-F | smm/hA3C-7 + 8 | AGATTTTAAATATTGTTGGGAAAACTTTGTG |
| HuSmmC7 + 8-R | CACAAAGTTTTCCCAACAATATTTAAAATCT | |
| HuSmmC9 + 10-F | smm/hA3C-9 + 10 | ATGAGCCATTCAAGCCTTGGAAGGGA |
| HuSmmC9 + 10-R | TCCCTTCCAAGGCTTGAATGGCTCAT | |
| RhA3CCE+QE-F | rhA3C-CE+QE | CAGTATCCGTGTTACCAGGAGGGGCTCCGCAGCCTGAGTCAGGAAGGAGTC |
| RhA3CCE+QE-R | GACTCCTTCCTGACTCAGGCTGCGGAGCCCCTCCTGGTAACACGGATACTG | |
| SmmA3CCE+QE-F | smmA3C-CE+QE | GGGATACATGTTACCAGGAGGGGCTCCGCAGCCTGAGTCAGGAAGGG |
| SmmA3CCE+QE-R | CCCTTCCTGACTCAGGCTGCGGAGCCCCTCCTGGTAACATGTATCCC | |
| HuA3CE106K-F | hA3C E106K | GGAGGTGGCCAAGTTCCTGGC |
| HuA3CE106K-R | GCCAGGAACTTGGCCACCTCC | |
| HuA3CQE-EK-F | hA3C QE-EK | AGCCTGAGTGAGAAAGGGGTCGCTG |
| HuA3CQE-EK-R | CAGCGACCCCTTTCTCACTCAGGCT | |
| HuA3CNQ+EK-F | hA3C NQ+EK | CAGTATCCAAATTACCAGCAGGGGCTCCGCAGCCTGAGTGAGAAAGGG |
| HuA3CNQ+EK-R | CCCTTTCTCACTCAGGCTGCGGAGCCCCTGCTGGTAATTTGGATACTG | |
| HuA3FDE-HQ-F | hA3F DE-HQ | CTGGGATACACATTACCAGCAGGGGCTC |
| HuA3FDE-HQ-R | GAGCCCCTGCTGGTAATGTGTATCCCAG | |
| HuA3FQE-EK-F | hA3F QE-EK | GCAGCCTGAGTGAGAAAGGGGCCTCCG |
| HuA3FQE-EK-R | CGGAGGCCCCTTTCTCACTCAGGCTGC | |
| HIV1Vif-EcorI-F | ATGAATTCGCCACCATGGAAAACAGATGGCAGG | |
| HIV1Vif-NotI-R | ATGCGGCCGCCTAGTGTCCATTCATTGTATGG | |
| F1D-G-NotI-R | F-1 D-G | ATGCGGCCGCCTAGTGTCCATTCATTGTATGGCTCCCTCTGTGGCCCTTGGTC |
| F1KD-RG-F | F-1K D-RG | AAGCCACCTTTGCCCAGTGTTA |
| F1KD-RG-R | TAACACTGGGCAAAGGTGGCTT | |
| F1K-R-F | F-1 K-R | GCCCAGTGTTAGGAAACTGACAGAG |
| F1K-R-R | CTCTGTCAGTTTCCTAACACTGGGC | |
| HIV1VIFRR-AA-F | B-NL4-3 RR-AA | GCAAGTAGACGCGATGGCGATTAACACATGG |
| HIV1VIFRR-AA-R | CCATGTGTTAATCGCCATCGCGTCTACTTGC | |
| HIV1VIFED-AA-F | B-NL4-3 ED-AA | GAAACTGACAGCGGCCAGATGGAAC |
| HIV1VIFED-AA-R | GTTCCATCTGGCCGCTGTCAGTTTC | |
| HIV1VIFD101G-F | B-NL4-3 D-G | CACAAGTAGACCCTGGCCTAGCAGACC |
| HIV1VIFD101G-R | GGTCTGCTAGGCCAGGGTCTACTTGTG |