FIG 2.

Group O Nefs cannot overcome BST2 restriction in viral spread assays. (A) Flow cytometry analysis of surface BST2 in control (MT4-shNT) or BST2-depleted (MT4-shBST2) cells. (B and C) MT4-shNT (B) and MT4-shBST2 (C) cells were infected with GFP-expressing NL4.3 WT and dU viruses expressing either no Nef (dN), NL4.3 Nef, or O-Nefs, as indicated (n = 3). A fraction of the cells was collected every day, and the percentage of GFP⁺ cells was evaluated by flow cytometry. Error bars represent standard deviations.