FIG 4.

Construction and characterization of UL37 mutant viruses. (A) Schematic representation of the UL37 mutant viruses constructed on the viral genomic background via double-Red mutagenesis on the HSV-1(F) and VC1 genomes cloned as a bacterial artificial chromosome. The approximate locations of the constructed mutations are shown. (B to E) Replication kinetics of wild-type and selected mutant viruses on Vero and BD45 cells. Viruses were adsorbed on nearly confluent monolayers of Vero or BD45 cells. Cells were infected with each virus at an MOI of 2. Virus stocks were collected at the indicated times, and titers were determined on both Vero and BD45 cells. Virus titers from three independent cultures were averaged, and the standard deviation was calculated for each time point.