FIG 3.

HSV-1 enters nTERT cells more rapidly than Vero cells. (A) Confluent layers of nTERT or Vero cells were infected with 200 PFU of HSV-1 per well of a 6-well plate on ice. After the indicated time, the inoculum was removed and replaced with medium containing 1% human serum. The infection was allowed to develop for 2 days, when plaques were fixed, stained, and counted. Data are represented as percent adsorption, where the final plaque count (at 180 min) is taken as 100%. The means ± standard errors of the data are given from one representative experiment (n = 3). (B) Confluent monolayers of nTERT or Vero cells were infected with 200 PFU of HSV-1 per well of a 6-well plate on ice for 2 h. The inoculum was removed and replaced with medium at 37°C and incubated for the indicated times before treatment with low-pH buffer for 1 min. After treatment, cells were washed and overlaid with medium containing 1% human serum. The infection was allowed to continue for 2 days, when plaques were fixed, stained, and counted. The means ± standard errors of the data are given from one representative experiment (n > 3). Data are displayed as the percentage of virus protected at each time, where the final plaque count (at 60 min postwarming) is taken as 100%. (C) Confluent monolayers of nTERT, Vero, or HFFF2 cells were infected with 200 PFU of HSV-1 and treated as described for panel B.