FIG 1.

Viral replication is dependent on the amino terminus of γ₁34.5. (A) Monolayers of HeLa cells were infected with wild-type HSV-1(F), R3616 (the γ₁34.5 null mutant), H1001(the γ₁34.5 mutant devoid of amino acids 1 to 146), or H1002 (the repaired virus with wild-type γ₁34.5) at 0.05 PFU per cell and incubated at 37°C. At the indicated time points postinfection, the total virus yields were determined on Vero cells. (B) HeLa cells were infected as described above. At 48 h postinfection, cell-associated viruses and viruses in the supernatant were collected separately and titrated on Vero cells. Data represent the mean values from three independent experiments with the standard deviations.