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Effect of γ134.5 variants on nuclear egress. Confluent monolayers of HeLa cell were infected with HSV-1(F), R3616, H1001, or H1002 at 5 PFU per cell. At 16 h postinfection, cells were harvested and processed for electron microscopy analysis as described in Materials and Methods. Abbreviations: Nuc, nucleus; Cyt, cytoplasm; NM, nuclear membrane. Scale bars are shown in each panel.
